Title : Investigating the effect of endurance exercise and two different food programs on experimental induced acute renal failure in male rats: the role of SIRT1
Abstract:
Objectives: Acute kidney injury (AKI) is a syndrome characterized by rapid loss of excretory function of kidney. Both exercise and some diets have been shown to increase silent information regulator (SIRT1) expression leading to reduction of kidney injury. In this study, the effect of alone and combined exercise and two different diets on kidney function, oxidative stress, inflammation, apoptosis and SIRT1 in AKI was investigated.
Methods: A number of rats were randomly divided into six groups; control, calorie restriction (CR), time restriction (TR), control with exercise, exercise + calorie restriction (CR), and exercise + time restriction (TR). Each group was divided into two subgroups of without AKI and with AKI (six rats in each group). Endurance exercise and diets were implemented before AKI. Serum urea and creatinine, urinary albumin, glomerular filtration rate (GFR), relative kidney weight, malondialdehyde (MDA), total antioxidant capacity (TAC), transforming growth factor (TGF-β1), SIRT1 levels, and Bcl2 Associated X (BAX), B-cell lymphoma 2 (Bcl2) levels, and histopathological outcome were measured before and 24 h after AKI induction.
Results: After induction of kidney injury, serum urea and creatinine, urinary albumin, relative kidney weight, kidney MDA and TGF-β1 levels, BAX increased in rats with both previous exercise and no previous exercise (p <0.001), while GFR, and kidney TAC SIRT1 and Bcl2 levels decreased (p <0.001). In the CR and TR group, these changes were less and in the TR group was greater. These changes after AKI were less in the group with previous exercise than in the group that had no exercise (p <0.001). The TR diet during exercise caused a less increase in serum urea (p <0.01) and creatinine (p <0.01), and urinary albumin (p <0.001) and BAX (p <0.001) levels after injury compared to the just exercise group. Also, both CR and TR diets during exercise caused less change in MDA (p <0.001) and TAC (p <0.05, p <0.001, respectively) and Bcl2 (p <0.001) levels compared to just exercise group.
Conclusion: The results showed that, both of exercise and diets alone caused less changes in function impairment of kidney, oxidative stress, inflammation, apoptosis and SIRT1 levels and histopathological outcome following AKI and the effect of TR is greater. However, when the CR and TR diets were implemented during exercise, strong renoprotective effects appeared. For the effectiveness of different diets, more research is needed according to the type, intensity, and duration of exercise.
Audience Take Away Notes:
- I try to share my latest findings with others and explain them clearly and the audience will find out what kind of diet to use to maintain their health to avoid the risk of metabolic diseases.
- They will also learn that the amount and type of diet and the intensity, type, and frequency of exercise are also involved.
- The results of this study also help nutritionists and exercise professionals to present a better and safety plan for maintaining of health to people.
